TABLE 3.

Diagnosis performance

	Sensitivity %	Specificity %
N‐Latex	93.8 (87.0–97.1)	95.8 (86.0–99.3)
ELISA	86.4 (78.2–91.9)	97.9 (89.1–99.9)
sIFE	64.6 (54.6–73.4)
uIFE	67.7 (57.8–76.2)
N‐Latex + sIFE	96.9 (91.2–99.2)
ELISA + sIFE	95.8 (89.8–98.4)
N‐Latex + sIFE + uIFE	100 (96.2–100)
ELISA + sIFE + uIFE	95.8 (89.8–98.4)

Note: Percent sensitivity and specificity (with 95% confidence interval) were calculated using κ/λ ratio reference intervals reported in Table 1. Sensitivity was also calculated for the detection of mFLC by immunofixation electrophoresis of the same serum (sIFE) and of a urine sample (uIFE) from the same patient, alone or in combination with FLC quantification. Due to the selection of patients without detectable monoclonal component in the serum or urine by EP and IFE for the control cohort (see material and methods), the specificity could not be calculated for these methods.