Extended Data Fig. 7 |. C34-CXCR4+ CAR T cells are selected for during chronic infection and exhibit superior ex vivo effector functions.

a, BLT mice were infected with HIV_JRCSF and 48 h later infused with 10⁷ C34-CXCR4⁺ Dual-CAR T cell product (TCP). FACS plots indicate the frequency of C34-CXCR4⁺ throughout infection. b, Mice were infected with HIV_MJ4 and 48 h later were infused with 10⁶ C34-CXCR4⁺ CAR.BBζ (n = 5), CAR.28ζ (n = 5), or purified Dual-CAR (n = 4) T cells. Frequency of C34-CXCR4⁺ CAR T cells in tissue 8 weeks post-infection. Thin dotted line indicates the frequency of C34-CXCR4⁺ CAR T cells in the pre-infusion TCP for the indicated CAR T cell type. Line and error bars indicate mean ± SEM. c, d, Mice were infected with HIV_MJ4 and 48 h later received 10⁶ C34-CXCR4⁺, purified CAR.BBζ.BBζ (n = 3), CAR.28ζ.28ζ (n = 4), or Dual-CAR (n = 3) T cells. c, FACS plots and (d) cumulative data show the frequency of each CD8⁺ CAR T cell population expressing MIP-1β and CD107a, and the frequency of CAR T cells with cytotoxic potential (granzyme B⁺ perforin⁺ CD107a⁺). CAR T cells were isolated from the spleen and bone marrow of mice 8 weeks post-infection and ex vivo stimulated. Significance was calculated using two-sided Wilcoxon matched-pairs signed rank test. For all data, symbols represent individual mice. Sample sizes in these studies indicate biologically independent animals.