Skip to main content
. 1999 Dec;181(24):7621–7625. doi: 10.1128/jb.181.24.7621-7625.1999

FIG. 4.

FIG. 4

puf mRNA decay in E. coli. After transferring plasmids pBT8 and pBRMOD11 into the E. coli strains N3433 (rne+) and N3431 [rne(Ts)], total RNA was isolated at different time points after addition of rifampin. An amount of 10 μg of total RNA in each lane was separated on a 1% agarose formaldehyde gel and transferred to a nylon membrane. After hybridization with the puf probe, we measured a half-life of 3 ± 0.5 (mean ± standard deviation) min for the 1.3-kb pufBAL/X mRNA in strain N3433(pBT8), and a half-life of 13 ± 2 min was determined for the same mRNA segment in strain N3431(pBT8). The 1.3-kb mRNA species in strain N3433(pBRMOD11) decayed with a half-life of 1.5 ± 0.5 min, whereas this mRNA segment of strain N3431(pBRMOD11) degraded with a half-life of 5 ± 2 min.