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Effect of resuscitation medium (ESPSN) on the resuscitation of M. tuberculosis cultures of varying ages, determined by FDA-EB staining
| Culture age (mo) | % of green (live) cells
|
||
|---|---|---|---|
| Direct staining | Fresh 7H9 medium (control) | ESPSN | |
| 8 | 1.6 | 9.4 | 49.8 |
| 10 | 1.8 | 2.4 | 49.2 |
| 15 | 0a | 0.8 | 19.4 |
| 16 | 0a | 0a | 11.2 |
All cells were orange-red.
