TABLE 2.

Growth stimulation of 6-month-old M. tuberculosis cultures by ESPSN with small bacterial inocula

Dilution	Visible growtha		CFU/mlb
	Sauton's	ESPSN	Sauton's	ESPSN
10−1	+	+	5 × 106	>107
10−2	+	+	106	107
10−3	−	+	ND	3 × 105
10−4	−	+	ND	2 × 105
10−5	−	−	ND	3 × 104
10−6	−	−	ND	ND

^a+, visible growth; −, no visible growth. 

^bDetermined from 2-week-old subcultures which had been inoculated with various dilutions of the 6-month-old culture diluted and grown in Sauton's medium alone or in ESPSN. Data are the averages of the results from triplicate platings of undiluted and diluted (10⁻²) samples on 7H11 agar plates. ND, no CFU detectable when directly plated on agar plates.