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Construction of vvh-lux fusion plasmid pYB9802. A PCR fragment carrying the regulatory region of the vvh operon was subcloned into pUCD615 carrying promoterless luxCDABE to create pYB9802. Filled blocks represent lux DNA, open blocks represent vvh DNA, and thin lines indicate the vector DNA used. Hybridizing locations of oligonucleotide primers used for PCR are depicted by open arrows. Abbreviations: B, BamHI; N, NdeI; P, PstI; S, SmaI.
