FIG. 3.

Dependency of hemolysin production of V. vulnificus on cAMP and CRP. (A) Cultures of strain MO6-24/O were grown in heart infusion broth with various supplements added as indicated. Samples removed at 12 h were analyzed for hemolytic activity. Error bars represent standard errors of the mean. (B) Growth and hemolytic activity of wild-type (●, ○) and crp mutant CMM988 (■, □) strains were compared. Samples removed at the indicated times from cultures were analyzed for determination of growth (filled symbols) and hemolytic activity (open symbols). For both panels, sRBC were used for determination of hemolytic activities. OD₆₀₀, optical density at 600 nm.