FIG. 3.

Lanes: a, SDS-PAGE pattern obtained by proteolytic degradation of the whole S-layer protein of B. sphaericus CCM 2177 with endoproteinase Gluc-C; b and e, proteolytic cleavage fragments that remained in the clear supernatant after incubation with native and HF-extracted peptidoglycan-containing sacculi, respectively; c and f, proteolytic cleavage fragments which could be removed from the bound fraction by washing native and HF-extracted peptidoglycan-containing sacculi, respectively, with buffer; d and g, proteolytic cleavage fragments which remained attached to native and HF-extracted peptidoglycan-containing sacculi, respectively. Molecular masses (in thousands) are shown at the left. Protein bands subjected to N-terminal sequencing are indicated by arrowheads.