TABLE 1.
Composition of native and HF-extracted peptidoglycan-containing sacculi of B. sphaericus CCM 2177a
| Amino acid or sugar | Molar composition
|
||
|---|---|---|---|
| Native | HF-extracted (48 h) | HF-extracted (96 h) | |
| GlcNH2 | 2.76 | 1 | 0.50 |
| Glu | 1 | 1 | 1 |
| Ala | 1.33 | 1.29 | 1.36 |
| Asp | 0.51 | 0.52 | 0.53 |
| Lys | 0.53 | 0.54 | 0.54 |
| ManNH2 | 0.82 | 0 | 0 |
a
For liberation of the peptidoglycan constituents hydrolysis was performed with 6 N HCl for 6 h at 110°C (9, 21, 24). Molar composition is shown with glutamic acid (Glu) = 1. GlcNH2, glucosamine; Ala, alanine; Asp, aspartic acid; Lys, lysine; ManNH2, mannosamine. (Muramic acid could not quantitatively be determined by this analysis method.)