TABLE 2.
NMR data of the pyruvate-free SCWP of B. sphaericus CCM 2177 recorded at 330 Ka
| Atom position | NMR analysis results for:
|
|||||
|---|---|---|---|---|---|---|
| →3)-β-ManNAc
|
→4)-β-GlcNAc
|
|||||
|
1H
|
13C chemical shift (ppm) |
1H
|
13C chemical shift (ppm) | |||
| Chemical shift (ppm) | J (Hz) | Chemical shift (ppm) | J (Hz) | |||
| 1 | 4.82 | 1.0 | 100.55 | 4.59 | 7.8 | 98.89 |
| 2 | 4.65 | 4.3 | 50.81 | 3.74 | ND | 56.14 |
| 3 | 4.03 | 9.8 | 77.99 | 3.70 | ND | 73.40 |
| 4 | 3.61 | 9.6 | 66.03 | 3.69 | ND | 80.06 |
| 5 | 3.46 | 2.4 | 77.25 | 3.52 | ND | 75.46 |
| 6 | 3.90, 3.78 | 12.2, 5.0 | 61.62 | 3.86, 3.80 | ND | 61.27 |
a
Other signals were as follows: 2.02 and 2.04 ppm (CH3), 23.38 and 23.07 ppm (CH3), and 175.31 and 175.41 ppm (CO). ND, not determined.