FIG. 2.

Determination of the transcription start sites of hrcA (A) and grpE (B) by primer extension mapping. Total RNA was isolated from B. japonicum 110spc4 cells harvested before (0) and 30 min after a heat shock from 30 to 43°C (30). The extension and sequencing reactions (TCGA) were performed with the primers HrcA11 (A) and GrpE4 (B). The transcription start sites are marked with arrows, and the deduced promoter sequences are shown below (C). Nucleotides matching those of the E. coli ς³² consensus promoter (52) and transcriptional start sites are in boldface.