FIG. 6.

Gel retardation experiments with purified B. japonicum HrcA. DNA-binding reactions were performed with DNA fragments derived from the promoter region of groESL₅ (A) and groESL₄ (A, C, and D). (B) groESL₄ fragments represented schematically. CIRCE₄* represents a DNA fragment in which the CIRCE element was mutated (see Materials and Methods). The amount of H₆-HrcA used is indicated above each lane in all panels. The positions of radiolabeled free DNA (f) and retarded bands (r) are marked.