TABLE 3.
Effect of an hrcA deletion on the expression of chromosomally integrated ′lacZ fusions in B. japonicum
| B. japonicum strainsa | ′lacZ fusion | Induction factorb | Mode of regulation |
|---|---|---|---|
| H8092/8092 | groEL5′-′lacZ | 2.3 ± 0.6 | CIRCE |
| H8548/8548 | groEL4′-′lacZ | 3.2 ± 0.7 | CIRCE |
| H8067/8067 | groEL1′-′lacZ | 1.2 ± 0.3 | RpoH |
| H8174/8174 | dnaK′-′lacZ | 0.9 ± 0.1 | RpoH |
| H5554/5554 | grpE′-′lacZ | 0.9 ± 0.1 | RpoH |
| H5559/5559 | hrcA′-′lacZ | 2.8 ± 0.6 | RpoH |
| H5400/5400 | hspA′-′lacZ | 1.1 ± 0.3 | ROSE |
| H7998/7998 | groEL2′-′lacZ | 1.3 ± 0.7 | Constitutive |
a
The indicated ′lacZ fusion (see also Table 1) was chromosomally integrated into wild-type B. japonicum (resulting strains without prefix) and into hrcA mutant 5549 (strains with prefix “H”).
b
The induction factor is the ratio of the measured β-galactosidase activity in the hrcA mutant to that for the wild-type background. Values are means ± standard deviations and are based on at least three independent measurements.