Table 1. Polymerase chain reaction primers used in this study.
Target genes | Primers | Primer Sequence (5’-3’) | Size (bp) | Reference |
---|---|---|---|---|
SFTSV L segment | SFTSV-L-F1 | AATGATGCCAAGAAGTGGAAT | 855 | This study |
SFTSV-L-R1 | ATGTAAGCATAGTCCTAGAAGC | |||
SFTSV-L-F2 | CCACAGATTCATTTGGGCT | 367 | ||
SFTSV-L-R2 | ATCATGATCGCTGAGTCGTC | |||
SFTSV M segment | SFTSV-M-F1 | TCTGCAGTTCAGACTCAGGGA | 761 | [38] |
SFTSV-M-R1 | GACGTGTATTGCTGTTTTCCC | |||
SFTSV-M-F2 | TGTTGCTTGTCAGCCTATGAC | 674 | ||
SFTSV-M-R2 | CAACCAATGATCCTGAGTGGA | |||
Mites COI | COI-F | GGTCAACAAATCATAAAGATATTGG | 710 | [36] |
COI-R | TAAACTTCAGGGTGACCAAAAAATCA | |||
Small mammals COI | COI-F | ACTTCTGGGTGTCCAAAGAATCA | 750 | [37] |
COI-R | CCTACTCRGCCATTTTACCTATG |
COI: mitochondrial cytochrome c oxidase subunit I gene