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. 2022 Aug 29;17(8):e0273029. doi: 10.1371/journal.pone.0273029

Table 1. Comparison of L, a* and b* values for images without any white balance correction (raw images).

L
Median (IQR) χ2 (df) P* a
Spectrophotometer (control) 62.45 (7.8) 226.691(5) p = 4.682 × 10−62
Photo box 71.50 (9.8)
Windowless clinic 1 73.00 (10.8)
Windowless clinic 2 57.00 (15.0)
Windowed clinic 1 72.00 (7.0)
Windowed clinic 2 61.00 (12.8)
b*
Median (IQR) χ2 (df) p* b
Spectrophotometer (control) 7.00 (5.1) 260.727 (5) P = 1.267 × 10−17
Photo box 20.50 (3.0)
Windowless clinic 1 7.00 (2.0)
Windowless clinic 2 8.00 (4.0)
Windowed clinic 1 10.00 (2.0)
Windowed clinic 2 9.00 (3.0)
b*
Median (IQR) χ2 (df) P* c
Spectrophotometer (control) 43.20 (10.7) 335.766 (5) p = 1.201 × 10−19
Photo box 42.00 (2.0)
Windowless clinic 1 22.00 (4.0)
Windowless clinic 2 21.00 (2.8)
Windowed clinic 1 27.00 (5.8)
Windowed clinic 2 24.00 (3.8)

*Significant < 0.05; χ2 = Chi-square statistics; df = degree of freedom, IQR = Interquartile Range

*Kruskal-Wallis one-way test: Parametric assumption not met. Shapiro- Wilk test significant (p < 0.05)

a Post-hoc analysis (Dunn’s test) for L: Null hypothesis rejected (p = 0.0 × 100 < 0.001). Spectrophotometer vs all other lighting conditions showed highly significant differences (p < 0.001) except windowed clinic 2 (p = 0.508). No significant difference was observed for photo box vs windowed clinic 1 (p = 0.303), photo box vs windowless clinic 1 (p = 0.258), and windowless clinic 1 vs windowed clinic 1 (p = 0.919)

b Post-hoc analysis (Dunn’s test) for a*: Null hypothesis rejected (p < 0.001). Spectrophotometer vs all other lighting conditions showed highly significant differences (p < 0.001) except windowless clinic 1 (p = 0.136). No significant differences observed for windowless clinic 1 vs windowless clinic 2 (p = 0.098) and windowed clinic 1 vs windowed clinic 2 (p = 0.208)

c Post-hoc analysis (Dunn’s test) for b*: Null hypothesis rejected (p < 0.001). Spectrophotometer vs all other lighting conditions showed highly significant differences (p < 0.001) except photo box (p = 0.985). No significant difference observed for windowless clinic 1 vs windowed clinic 2(p = 0.101)