Figure 3:

Quantitative methods commonly used with colorimetric assays on μPADs. (A) Calibration curve for intensity-based analysis of a scanned device using ImageJ. Reprinted with permission from Ref. 55. Copyright 2012 American Chemical Society. (B) Count-based quantitation achieved by counting the number of colored tabs. Adapted from Ref. 167 with permission from John Wiley and Sons. (C) Distance-based detection by measuring the length of color development along a channel. Reproduced from Ref. 68 with permission from The Royal Society of Chemistry. (D) Time-based quantification. Reproduced from Ref. 169 with permission from The Royal Society of Chemistry. (E) Use of a smartphone to conduct intensity-based measurements. Reprinted with permission from Ref. 88. Copyright 2014 American Chemical Society.