Table 2.

Promising Live Attenuated Vaccines developed in 2015–2022. PBMs porcine blood monocyte/macrophages; BMs pig bone marrow cells; COS-1 monkey kidney tissue-derived cells; PAMs primary porcine alveolar macrophages; PIPEC Plum Island porcine epithelial cells, a porcine fetal kidney cell line engineered to express the bovine αVβ6 integrin.

Candidate	ASFV strain	Virulence	p72 genotype	Attenuation strategy	Protection	Production system	References
NH/P68	NH/P68	High	I	Naturally attenuated	Homologous and heterologous strain (L60, Arm07)	PBMs	Gallardo et al., 2012; Leitão et al., 2001a
OURT88/3	OURT88/3	Low	I	Naturally attenuated	Homologous and heterologous strain (OURT88/1, Ug65)	BMs	Boinas et al., 2004b; King et al., 2011; Mulumba-Mfumu et al., 2016; Sánchez-Cordón et al., 2017
Lv17/WB/Rie1	Lv17/WB/Rie1	Low	II	Naturally attenuated	Homologous strain (Armo7)	PBMs	Barasona et al., 2019; Gallardo et al., 2019
BA71ΔCD2v	BA71	Low	I	Gene deleted (CD2v)	Homologous and heterologous strain (E75, Georgia 2007)	COS-1	Lopez et al., 2020; Monteagudo et al., 2017
HLJ/18-7GD	HLJ/18	High	II	Gene deleted (MGF505-1R, MGF360-12L, MGF360-13L, MGF360-14L, MGF505-2R, MGF505-3R, and CD2v)	Homologous strain (ASFV HLJ/18)	PAMs	Chen et al., 2020
ASFV-G-ΔI177L	Georgia 2007	High	II	Gene deleted (I177L)	Homologous strain (Georgia 2007)	PAMs	Borca, Ramirez-Medina, et al., 2020
ASFV-G-ΔI177L/ΔLVR	ASFV-G-ΔI177L	High	II	Gene deleted (I177L) and cell passage	Homologous strain (Georgia 2007)	PIPEC	Borca, Rai, et al., 2021
SY18ΔI226R	ASFV-SY18	High	II	Gene deleted (I226R)	Homologous strain (ASFV-SY18)	PAMs	Zhang et al., 2021
ASFV-G-ΔA137R	Georgia 2010	High	II	Gene deleted (A137R)	Homologous strain (Georgia 2010)	PAMs	Gladue et al., 2021
ASFV-G-ΔE184L	Georgia 2010	High	II	Gene deleted (E184L)	Homologous strain (Georgia 2010)	PAMs	Ramirez-Medina et al., 2022