Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Construction and validation of TRIM50 shRNA and overexpressing GC cells. TRIM50 mRNA and protein expression in GC cells transfected with shTRIM50 (a, b) or pLVX-Puro-TRIM50 (c, d) was measured with Western blot and qPCR after 48 h of transfection. One-way ANOVA and Tukey tests were applied for multi-group comparison, and two-tailed Student's-test was utilized for intergroup comparisons. ▲p < 0.05 and ▲▲p < 0.01vs. shNC or Vector. Results were presented as mean ± SD. n = 3.
