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TRIM50 affected the expression of the cell cycle-related proteins in GC cells. The expression of p21, p27, CDK4, and Cyclin D1 proteins in GC cells transfected with pLVX-Puro-TRIM50 (a) or shTRIM50 (b) was detected by Western blot after 48 h of transfection. Two-tailed Student's-test was utilized for intergroup comparisons. ▲p < 0.05 and ▲▲p < 0.01vs. Vector or shNC. Results were presented as mean ± SD. n = 3.
