Figure 7.

TRIM50 could interact with β-catenin, induce β-catenin degradation. (a) After 48 h of transfection, the levels of the β-catenin, c-Myc, survivin, and Cyclin D1 proteins in GC cells were quantified using Western blot. (b) The interaction between TRIM50 and β-catenin was tested with Co-IP analysis. (c, d) The effect of TRIM50 overexpression on β-catenin degradation was tested with the treatment of CHX (200 μg/ml) and MG132 (10 μM). One-way ANOVA and Tukey tests were applied for multi-group comparison, and two-tailed Student's-test was utilized for intergroup comparisons. ^▲p < 0.05 and ^▲▲p < 0.01vs. Vector or shNC. Results were presented as mean ± SD. n = 3.