Table 2.
Antibody | Host | Dilution | Company, catalogue #, and RRID |
---|---|---|---|
Alexa Fluor 488 AffiniPure Donkey Anti-Rabbit IgG | Donkey | 1 : 200 | Jackson ImmunoResearch Laboratories, Inc. 711-545-152, RRID AB_2313584 |
Alexa Fluor 594 AffiniPure Donkey Anti-Mouse IgG (H + L) | Donkey | 1 : 200 | Jackson ImmunoResearch Laboratories, Inc., 715-585-150, RRID AB_2340854 |
Anti-AFP | Rabbit | 1 : 200 | GeneTex GTX72748, RRID_374821 |
Anti-beta (β)-actin | Mouse | 1 : 1000 | Cell signaling technology (CST), cat# 4967 |
Anti-C3 | Rabbit | 1 : 100 | ABclonal A13283, RRID AB_2760135 |
Anti-GFAP | Mouse | 1 : 200 | BD Pharmingen cat# 556327, RRID AB_396365 |
Anti-LC3B | Rabbit | 1 : 1000 | Gene Tex, cat# GTX127375 |
Anti- mTOR (7C10) | Rabbit | 1 : 1000 | Cell signalling technology (CST), cat# 2983 |
Anti-NANOG | Rabbit | 1 : 200 | Abcam ab21624, RRID: AB_446437 |
Anti-nestin | Mouse | 1 : 200 | Gene Tex cat# GT935, AB_2716635 |
Anti-OCT4 | Rabbit | 1 : 200 | Abcam cat# ab18976, RRID: AB_444714 |
anti-phospho-mTOR (Ser 2448) | Rabbit | 1 : 1000 | Cell signaling technology (CST), cat# 2971 |
Anti-SOX2 | Rabbit | 1 : 200 | Abcam ab97959, RRID: AB_2341193 |
Anti-SMA | Mouse | 1 : 200 | Dako M0851, RRID AB_2223500 |
Anti-S100β | Rabbit | 1 : 100 | Gene Tex, GTX129573, RRID AB_2886037 |
Anti TRA-1-60 | Mouse | 1 : 200 | Abcam ab16288, RRID: AB_778563 |
Anti-TUJ | Rabbit | 1 : 200 | Abcam, T2200, RRID: AB_10704920 |
Anti-Ulk1 | Rabbit | 1 : 1000 | GeneTex, cat # GTX16974 |
Peroxidase-conjugated AffiniPure Goat Anti-Mouse IgG (H + L) | Goat | 1 : 10000 | Jackson ImmunoResearch Laboratories, Inc. 115-035-003, RRID: AB_10015289 |
Peroxidase-conjugated AffiniPure Goat Anti-rabbit IgG (H + L) | Goat | 1 : 10000 | Jackson ImmunoResearch Laboratories, Inc. 111-035-144, RRID: AB_2307391 |
DAPI | 1 : 1000 | Sigma, D9542 |