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. 2022 Aug 22;2022:1614336. doi: 10.1155/2022/1614336

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Copyright © 2022 Ge Zhang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Hypoxic M2 macrophage-derived conditional medium facilitates GBM aggressiveness and angiogenesis in a VEGF-dependent manner. (a) The graphical illustration for the coculture of GBM cells and the macrophage-derived conditional medium. (b, c) GBM cell proliferation abilities were determined by performing MTT assay. (d, e) The cell mitosis-associated biomarkers (CDK2, CDK6, and Cyclin D1) were examined by performing Real-Time qPCR analysis. (f, g) The EMT-associated signatures (N-cadherin, Slug, Twist, and E-cadherin) were examined by Real-Time qPCR analysis. Each experiment repeated at least 3 times, and ^∗P < 0.05.