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M2-CM VEGF-dependently increased TMZ resistance in the GBM cells. (a, b) MTT assay results verified that M2-CM increased cell proliferation in the GBM cells cotreated with TMZ. (c, d) FCM assay verified that M2-CM suppressed TMZ-induced apoptotic cell death in the GBM cells. (e, f) Real-Time qPCR and (g, h) Western Blot analysis were used to examine the expression status of GSC-related proteins (CD133, OCT4, and Nanog). Each experiment repeated at least 3 times, and ∗P < 0.05.
