Figure 4.

MITD1 knockdown induces ferroptosis through downregulating SLC7A11. (a) Western blot of MITD1and ferroptosis-related proteins (GPX4, SLC7A11, COX2, and ACSL4) in ccRCC cells after silencing MITD1 (SiMITD1). (b, c) 786-O cells or A498 cells were transfected with TAZ plasmid after silencing MITD1. Western blot of MITD1 and SLC7A11. (d–f) Levels of MDA, GSH, and SOD in ccRCC cells with different treatments. Values are expressed as the mean ± SEM, n = 3. ^∗P < 0.05, relative to the control group; ^#P < 0.05, relative to the SiMITD1.