Figure 6.

MITD1 knockdown induces ferroptosis through the TAZ/SLC7A11 pathway. (a, b) 786-O cells or A498 cells were transfected with negative control or si-RNA for MITD1 (SiMITD1) and then were transfected with TAZ plasmid. Western blot of MITD1, SLC7A11, and TAZ. (c) Representative images of 786-O cells with DCFH-DA staining (magnification ×100; scale bars = 100 μm) after different treatments. (d–f) Levels of MDA, GSH, and SOD in ccRCC cells with different treatments. Values are expressed as the mean ± SEM, n = 3. ^∗P < 0.05, relative to the control group; ^#P < 0.05, relative to the SiMITD1.