FIG. 3.

Thin-layer chromatogram of quinone standards and of quinones isolated from S. putrefaciens cells grown anaerobically with TMAO as the electron acceptor. Lanes 1 and 2 were loaded with ubiquinone-6 and menaquinone-4 standards, respectively. The other lanes were loaded with quinone extracts (equivalent to 0.10 g of wet cells) isolated from cells of the following strains: lane 3, MR-1; lane 4, MR-1/pVK100; lane 5, MR1-CYMA/pVK100; lane 6, MR1-CYMA/pCMTN1-VK; lane 7, MR-1A; lane 8, CMTn-1/pVK100; lane 9, CMTn-1/pCMTN1-VK. The samples were loaded at the bottom, and migration was upward. The quinone standards did not exactly migrate with the quinones from S. putrefaciens because the length and composition of the isoprenyl side chain affect the migration. In a previous report (32), spots I and II were identified as methylmenaquinone and menaquinone, respectively, because of their migration relative to the identified quinones in S. putrefaciens ATCC 8071 (14). Spots III, IV, and V were identified as ubiquinones by a similar comparison.