Table 3.
Multivariable survival analysis of intraepithelial CD8 and triple-positive Treg scores.
| Multivariable analysis c-index (concordance): 0.695 (se = 0.013) | |||
|---|---|---|---|
| Statistic | HR | 95% CI | p value |
| ieCD8 | |||
| High vs low | 0.62 | 0.50–0.76 | <0.0001 |
| Mean CD25 expression in tp-Tregs | |||
| High vs Low/tp-TregNegative | 1.35 | 1.03–1.75 | 0.028 |
| CD8-tp-Treg spatial proximity | |||
| Significant vs non-significant | 1.36 | 1.06–1.75 | 0.017 |
| pT | |||
| T3 vs T1/2 | 1.50 | 1.14–1.98 | 0.0038 |
| T4 vs T1/2 | 3.00 | 1.93–4.64 | <0.0001 |
| pN | |||
| N1 vs N0 | 1.54 | 1.21–1.97 | 0.00050 |
| N2 vs N0 | 2.34 | 1.72–3.18 | <0.0001 |
| MSI status | |||
| MSI vs MSS | 0.72 | 0.54–0.96 | 0.027 |
| Age | 1.04 | 1.03–1.05 | <0.0001 |
The variables selected by bootstrap sampling and backwards selection were included in multivariable survival analysis of the combined Norwegian series. Analysis using the continuous score for intraepithelial CD8 is presented in Supplementary Table 15 and analysis including the stromal CD3 score is presented in Supplementary Table 16. The analysis was stratified by cohort and performed within stage I–III, R0 CRC patients. Endpoint evaluated was 5-year relapse-free survival. Only patients with complete data for all variables were included in the analysis.
n = 977, events = 376.
ieCD8 intraepithelial CD8, MSI microsatellite instable, MSS microsatellite stable, tp-Tregs triple-positive Tregs.