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. 2022 Aug 29;13(8):748. doi: 10.1038/s41419-022-05169-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Expression and the correlation of CD163 and EZH2 were examined by IHC in breast cancer patients from a tissue microarray (n = 139). Scale bar, 50 μm. The percentage of total macrophages, M1 and M2 macrophages in 200 mg/kg/day EPZ-6438-treated PDX6305 tumor tissues B and BT549 EZH2-knockdown tumor tissues C were analyzed by flow cytometry. RT-qPCR was applied to detect the expressions of M1 markers (iNOS, Il1b, TNFα) and M2 markers (CD206, Arg1, Ym1) in EPZ-6438-treated PDX6305 tumor tissues D or BT549 EZH2-knockdown tumor tissues E. F Immunohistochemistry of F4/80, CD163 and H3K27me3 in tumor tissues treated with or without EPZ-6438. G Immunohistochemistry of F4/80, CD163 and EZH2 in mock and EZH2 knockdown tumor tissues. Scale bar, 50 μm. In B–E, all data were shown as mean values ± SD (n = 3). Statistical significance was addressed using unpaired, two‐tailed Student’s t‐test, *p < 0.05, **p < 0.01, ***p < 0.001, NS, not significant. The statistical significance was calculated compared to the negative control (NC) in each group. The shown were representative of replicates and the experiments were repeated three times.