Figure 1.

Brain cancer cells invading the ECM. (A) Schematics of an ECM-embedded spheroid in a gradient assay (GA) or individual cells in a gradient-free assay (GFA). (B) Tracks of migrating cells in a GA from $t=9\text{h}$ and $t=5\text{h}$, respectively, superimposed on the corresponding bright field image. For each track, the color bar indicates time evolution. Scale bar is $100\mathrm{\mu }\text{m}$. (C) Morphological transition of a cell prior to migration. Image sequence of brain cancer cells invading the ECM at $t=\{0,15,45,120\}$ min in GFA. The track of the cell is superimposed on the bright field image. The color bar indicates time evolution and the scale bars are $50\mathrm{\mu }\text{m}$.