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. 2022 Aug 29;8:376. doi: 10.1038/s41420-022-01169-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A, B GSH and Fe²⁺ content in liver cancer cells with or without HERPUD1 after adding erastin, RSL3, CA. C The ferroptosis-related metabolic axis from glucose to GSH. D mRNA levels of GCLC, GSS, CBS, CTH, and SHMT2 were analyzed by qPCR in Bel-7402 and Bel-7404 cells with or without HERPUD1. E Protein levels of GCLC, GSS, CBS, CTH, and SHMT2 were analyzed by immunoblotting in Bel-7402 and Bel-7404 cells with or without HERPUD1. F The relative protein level of GCLC, GSS, CBS, CTH, and SHMT2 are normalized according to the level of GAPDH calculated by ImageJ software. The data were analyzed using Student’s t test and shown as the mean ± SD from three biological replicates. *P < 0.05; **P < 0.01; ***P < 0.001 indicates statistical significance.