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. 2022 Aug 29;13(8):746. doi: 10.1038/s41419-022-04956-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Schematic diagram for prediction of 14 candidate circRNAs that were both related to AR target-genes (FKBP5 and PSCA) B qRT-PCR was performed to show quantification of 14 candidate circRNAs expression after 50 nM DHT treatment in EnzS1-C4-2 (left panel) and EnzR1-C4-2 (right panel) cells. C Western blot showed PD-L1 expression after sh-circ76151 (left panel), sh-circ127664 (right panel) in both cell lines and treated with 50 nM DHT or EtOH. D Before treating EnzS1-C4-2 cells with 50 nM DHT, circ76151 (left panel) and circ127664 (right panel) expressions were knocked down. After treatment, MTT assay was performed separately using NK92-MI cells to target EnzS1-C4-2 cells. E RNase R assay to determine the sensitivity of circFKBP5 by RNase R digestion in EnzS1-C4-2 (left panel) and EnzR1-C4-2 (right panel). F qRT-PCR was applied to test FKBP5 mRNA expression in EnzS1-C4-2 (left panel) and EnzR1-C4-2 (right panel) after 50 nM DHT treatment for 48 h. G qRT-PCR was performed to evaluate the efficacy of knocking down FKBP5 (shFKBP5) in EnzS1-C4-2 (left panel) and EnzR1-C4-2 (right panel) with/without (w/wo) shFKBP5. H Western blot showed PD-L1 expression in both cell lines after sh-FKBP5 and treated with 50 nM DHT or EtOH. *p < 0.05 **p < 0.01, NS = Not Significant.