Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Aug 29;13:5070. doi: 10.1038/s41467-022-32675-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Illustration of chromatin configuration evolution (non-surrounded nucleolus (NSN), transitioning (Trans), surrounded nucleolus (SN)) coinciding with oocyte growth subcategories (mid-antral follicle, late-antral follicle, and fully grown); (left) large spherical nucleoli decrease in number and increase in size as of the Trans-stage; (right) chromatin configuration scenarios in fully grown cytoplasmic F-actin mutants (FMN2^−/−) with one large or two smaller nucleoli. The nuclear membrane (gray) is depicted according to fluctuation intensity. b Top: Bright-field images of Control oocytes with growth progression and a fully grown mutant; the nucleus is in dashed white and orange arrowheads indicate multiple small nucleoli (NSN and FMN2^−/− SN) or a single large nucleolus (Trans and SN). Bottom: Growth-associated cytoplasmic stirring vector maps generated by STICS analyses of bright-field 240 s-stream videos; the oocyte cortex is outlined with colors reflecting the intensity of cytoplasmic stirring; maps are color-coded according to velocity magnitude (lowest in dark blue, strongest in white). c Cytoplasmic random stirring intensity in time measured in Control NSN to SN and FMN2^−/− SN oocytes by image correlation analyses of cytoplasmic pixel evolution; color gradient (left) used in this study to represent cytoplasmic stirring intensity; cell number, Control NSN = 22, Trans = 19, SN = 28 cells, FMN2^−/− SN = 13; error bars represent mean-s.d. d Representative immunoreactivities of Coilin and nuclear speckles in growing Control oocytes and SN FMN2^−/− oocytes; Coilin images are 0.5 μm z-planes and nuclear speckle images are 20 μm z-projections; nucleus regions outlined with dashed circles. e Quantifications of Coilin (top) and nuclear speckle (bottom) droplet number and surface in nuclei of fully grown SN Control and mutant oocytes; Coilin, counted in 43 Control and 17 FMN2^−/− cells, measured 44 Control and 33 FMN2^−/− droplets; speckles, counted in 41 Control and 52 FMN2^−/− cells, measured 62 Control and 112 FMN2^−/− droplets; violin plots with median ± quartiles. P values derived from two-tailed Mann–Whitney U-tests, ns not significant, P = 0.074, ***P < 0.0004, ****P < 0.0001; color codes based on cytoplasmic stirring intensities; scale bars, 5 μm. Source data are provided as a Source Data file.