Fig. 4.

IDF-11774 downregulates EMT in B16F10 melanoma. (A, B) Western blot analysis of E-cadherin, N-cadherin and SNAIL levels (A) and representative immunofluorescent staining using anti-N-cadherin or anti-SNAIL antibody (B) in cultured B16F10 treated with IDF-11774 in the absence or presence of CoCl2 for 48 h. (C, D) Representative immunofluorescent staining of B16F10 melanoma in nude mice treated without (control) or with 60 mg/kg/day of IDF-11774 using anti-N-cadherin (C) or anti-SNAIL antibody (D). β-Actin was used as an internal control for Western blot analysis. Nuclei were counter-stained with Hoechst 33258 (Bar=0.05 mm). Intensities of immunofluorescence staining were measured using a Wright Cell Imaging Facility ImageJ software. Data in the graph represent mean ± SD of relative values compared to non-treated control from four independent experiments. *p<0.05, **p<0.01.