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Immunohistochemistry of ALES. The tumor cells were strongly and diffusely positive for AE1/AE3 (A), p40 (B), CD99 (C), and NKX2.2 (D). Fluorescent in situ hybridization revealed EWSR1 rearrangement (red-green ‘spilt’ signals in the inset, E E–F; DAPI × 1000)
