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Localization of put genes of P. putida KT2442 in vectors pLCR4, pLCR12, and pSLH4. The pLCR4 plasmid contains 2 kb of the putA gene, and pLCR12 contains 450 bp of the putA gene, all the putP genes, and the intervening regulatory region between the two genes that are transcribed divergently. A 12-kb insert in plasmid pSLH4 bears the complete proline utilization operon.
