TABLE 1.
Bacteria and plasmids used
| Strain or plasmid | Relevant characteristic(s)a | Reference or source |
|---|---|---|
| Strains | ||
| P. putida | ||
| KT2442 | Rifr Apr Cmr; prototroph | 18 |
| S14D2 | Rifr KmrputA::mini-Tn5 luxAB-Km | This study |
| E. coli | ||
| HB101 | SmrrecA | 5 |
| DH5αF′ | recA | 62 |
| CC118λpir | Rifr; λ-pir lysogen | 22 |
| RM2 | Δ(putA putP) | 20 |
| Plasmids | ||
| pCK220 | Apr Kmr mini-Tn5::′luxAB | 52 |
| pCRR831 | Tcr; chimeric cosmid of P. putida library bearing the proline utilization operon | C. Ramos and L. Molina |
| pMIS5 | TcrPputA::′lacZ oriRK2 | This study |
| pMIS12 | TcrPputP::′lacZ oriRK2 | This study |
| pMP220 | Tcr ′lacZ oriRK2 | 50 |
| pPC6 | Apr; 6-kb AatII-PvuII fragment from the serovar Typhimurium chromosome in pBR322 | 20 |
| pRK600 | Cmrmob+ tra+ | 22 |
| pUC18/19 | Apr; cloning vector | 57 |
a
Apr, Rifr, Kmr, Cmr, Smr, and Tcr indicate resistance to ampicillin, rifampin, kanamycin, chloramphenicol, streptomycin, and tetracycline, respectively.