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PDH activity of P. putida KT2442 and its mini-Tn5 transposon derivativesa
| Strain | PDH activity (%)
|
|
|---|---|---|
| −Pro | +Pro | |
| KT2442 | 5 | 100 |
| S14D2 | 1 | 5 |
| S14D2(pCCR831) | 3 | 72 |
Cells were grown on M9 minimal medium with succinate as the sole C source in the absence (−Pro) and the presence (+Pro) of 20 mM proline. PDH activity was determined as described in Materials and Methods. One hundred percent of activity corresponded to 190 nmol of P5C produced per milligrams of protein per minute.
