TABLE 3.
Expression from the put promoters in P. putidaa
| Strain and fusion | Growth conditions | β-Galactosidase activity |
|---|---|---|
| Wild type | ||
| PputP::′lacZ | Succinate | 700 ± 50 |
| Succinate plus proline | 2,800 ± 100 | |
| PputA::′lacZ | Succinate | 350 ± 30 |
| Succinate plus proline | 6,950 ± 100 | |
| Mutant S14D2 | ||
| PputP::′lacZ | Succinate | 2,700 ± 200 |
| Succinate plus proline | 2,600 ± 150 | |
| PputA::′lacZ | Succinate | 7,900 ± 200 |
| Succinate | 8,600 ± 250 |
a
P. putida KT2442 and P. putida S14D2 bearing pMIS5 (PputA::′lacZ) or pMIS12 (PputP::′lacZ) were grown on M9 minimal medium with succinate or succinate plus proline. β-Galactosidase activity (in Miller units) was determined in permeabilized whole cells according to the method of Gallegos et al. (19). Data are the average of four independent assays.