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. 2000 Jan;182(1):91–99. doi: 10.1128/jb.182.1.91-99.2000

TABLE 4.

Induction of the put promoters in the presence of corn root exudatesa

Fusion Incubation conditions β-Galactosidase activity
PputA::′lacZ Corn exudate 425 ± 50
M8 20 ± 10
PputP::′lacZ Corn exudate 270 ± 50
M8 60 ± 10
a

P. putida KT2442 cells bearing pMIS5 (PputA::′lacZ) or pMIS12 (PputP::′lacZ) grown on M9 minimal medium with succinate were harvested and suspended in M8 minimal medium (M8) or in the same medium enriched for 7 days with corn root exudates (corn exudate) and incubated at room temperature without agitation. β-Galactosidase activity was determined as described in the footnote to Table 3