TABLE 4.
Fusion | Incubation conditions | β-Galactosidase activity |
---|---|---|
PputA::′lacZ | Corn exudate | 425 ± 50 |
M8 | 20 ± 10 | |
PputP::′lacZ | Corn exudate | 270 ± 50 |
M8 | 60 ± 10 |
P. putida KT2442 cells bearing pMIS5 (PputA::′lacZ) or pMIS12 (PputP::′lacZ) grown on M9 minimal medium with succinate were harvested and suspended in M8 minimal medium (M8) or in the same medium enriched for 7 days with corn root exudates (corn exudate) and incubated at room temperature without agitation. β-Galactosidase activity was determined as described in the footnote to Table 3.