TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| E. coli strains | ||
| DH5α | F− λ−recA1 Δ(lacZYA-argF)U169 hsdR17 thi-1 gyrA96 supE44 endA1 relA1 φ80dlacZΔM15 | GIBCO-BRL |
| BL21(DE3) | Carries T7 RNA polymerase under the control of the lacUV5 promoter | 34 |
| S17-1 λpir | S17-1 lysogenized with λpir phage | 32 |
| RZ4500 | ΔlacZ | 6 |
| RZ8459 | ΔlacZ ΔfnrΩSpr | P. Kiley |
| P. aeruginosa PAO1 | Wild-type strain | A. Kropinsky |
| R. palustris strains | ||
| CGA009 | Wild-type strain; spontaneous Cmr derivative | 21 |
| CGA507 | hbaA::′lacZ | This study |
| CGA612 | hbaR | This study |
| CGA614 | hbaA::′lacZ hbaR | This study |
| Plasmids | ||
| pUC19 | High-copy-number cloning vector; Apr | 40 |
| pJQ200mp18 | Mobilizable suicide vector; sacB; Gmr | 28 |
| pCF116 | Mobilizable suicide vector; sacB; Kmr | 14 |
| pUCGM | Contains Gmr cassette | 31 |
| pBBR1MCS-2 | Broad-host-range vector; Kmr | 22 |
| pT7-7 | T7 promoter expression vector; Apr | 35 |
| pHRP309 | Reporter plasmid, contains ′lacZ | 26 |
| pHRP311 | pHRP309 with ΩSpr cassette cloned in front of ′lacZ | 26 |
| pHRP315 | Cohort vector for pHRP309 cloning system; Apr Spr | 26 |
| pHRP316 | Cohort vector for pHRP309 cloning system; Apr Spr | 26 |
| pPE604 | pUC19 with a 1.9-kb PCR product containing hbaR | This study |
| pPE604::Gmr | pPE604 with Gmr cassette from pUCGM at the AgeI site of hbaR | This study |
| pPE900 | pCF116 containing the hbaR::Gmr construct from pPE604::Gmr at the XbaI site | This study |
| pPE901 | pBBRMCS-2 with the hbaR-containing XbaI fragment from pPE604 | This study |
| pPE905 | pT7-7 with hbaR under the control of the T7 promoter | This study |
| pPE906 | pHRP309 with the ΩSpr and hbaR promoter region fused to ′lacZ; Spr Gmr | This study |
| pMD300 | Contains the 2.1-kb EcoRI fragment containing hbaA | 16 |
| pMD425 | pJQ200mp18 with a 2.1-kb hbaA-containing fragment | This study |
| pMD426 | pJQ200mp18 with a 2.1-kb hbaA-containing fragment interrupted with ′lacZ-Kmr at a unique SalI site | This study |
| pMD505 | pHRP309 with a ΩSpr and hbaA promoter region fused to ′lacZ; Spr Gmr | This study |
a
Apr, ampicillin resistance; Kmr, kanamycin resistance; Gmr, gentamicin resistance; Cmr, chloramphenicol resistance; Spr, spectinomycin resistance; MCS, multiple cloning site.