a) Schematic representations of berberine coated with (2-Hydroxypropyl)- β-cyclodextrin. b) The absorption and emission spectra of berberine@(2-Hydroxypropyl)-β-cyclodextrin. c) The chemical formula of ICG. d) The absorption and emission spectra of ICG solution. e) Comparison of the in vitro performance of ICG, berberine, and mixture, under different excitation conditions. The performances of the mixed tracer f) in an ICR mouse, g) in a rat and h) in a rabbit (scale bar: 1 cm). NIR-II imaging condition: 1100 long-pass filter, exposure time, 10 ms; power intensity, 15 mW/cm2.