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. 2022 Aug 16;9:986943. doi: 10.3389/fnut.2022.986943

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Copyright © 2022 Lv, Huang, Li, Gong, Sun, Mao and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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POAE and POEE reduced the density of nerve fibers in the dorsal skin. (A) Representative photographs of H&E staining of the dorsal skin of each group of mice (Scale bar: 200 μm). The black arrow indicates the epidermal layer of the skin. (B) Thickness of the epidermal layer of the dorsal skin of each group of mice (n = 3 per group). (C) Immunofluorescence staining of representative dorsal skin nerve fibers from each group of mice (Scale bar: 50 μm). The nuclei showed blue fluorescence after DAPI staining, and PGP9.5 showed red fluorescence under the labeling of fluorescent secondary antibody. (D) The ratio of PGP9.5/DAPI fluorescence area in each group of mice (n = 4 per group). The data were expressed as mean ± SD. ^###P < 0.001, vs. control groups; **P < 0.01, ***P < 0.001, vs. DNCB (model) groups.