FIG. 2.
Steady-state levels of GFP-FtsL-MalF swap proteins as determined by Western blotting with an anti-FtsL antibody. Molecular mass standards (kilodaltons) are indicated to the left of the blot, while the positions of GFP-FtsL fusions are shown to the right. The GFP fusion protein produced is indicated above each lane. Strains used were as follows: (A) GFP, EC452; GFP-FtsL, EC438; GFP-LLL, JMG287; GFP-LFL, JMG289; GFP-FFL, JMG292; GFP-FLL, JMG469; GFP-LHL, JMG372; GFP-HLL, JMG371; and GFP-HHL, JMG374; (B) GFP, EC452; GFP-FtsL, EC438; GFP-LLL, JMG287; GFP-LLΔL, JMG299; GFP-LLL(L70H), JMG303; GFP-LLL(L84D), JMG304; GFP-LLHinfL, JMG300; GFP-LLGCN4L, JMG301; and GFP-LLC/EBPL, JMG302.