TABLE 1.
Bacterial strains used
| Strain | Relevant genetic markers or features | Constructiona | Source or reference |
|---|---|---|---|
| Strains without gfp fusions | |||
| KS272 | F− ΔlacX74 galE galK thi rpsL ΔphoA(PvuII) | Laboratory collection | |
| MC4100 | F−araD139 ΔlacU169 relA1 rpsL150 thi mot flb5301 deoC7 ptsF25 rbsR | Laboratory collection | |
| JP313 | MC4100 Δara714 | J. Pogliano | |
| LMG145 | KS272 ftsL::TnphoAL81ΔIS50R(Kanr)/pLMG180 | 9 | |
| JMG47 | KS272 ftsL::TnphoAL81ΔIS50R(Kanr)/pLD45 | P1 on LMG145 × KS272 pLD45, selecting for Kmr | This study |
| DHB4 | F− Δlacpro lacIq/Δ(malF) ΔphoA (PvuII) phoR ΔlacX74 Δ(ara leu)7697 araD139 galE (or U) galK Strr | D. Boyd | |
| DHB9 | DHB4 recA1 srl::Tn10 | D. Boyd | |
| JMG48 | KS272 ftsL::TnphoAL81ΔIS50R(Kanr)/pJMG64 | P1 on LMG145 × KS272 pJMG64, selecting for Kmr | This study |
| JMG33 | KS272 ftsL::TnphoAL81ΔIS50R(Kanr) recA1 srl::Tn10/pJMG64 | P1 on DHB9 × JMG48, selecting for Tetr | This study |
| DHB6521 | SM551 λInCh (Kanr) | D. Boyd et al., unpublished data | |
| Depletion backgrounds | |||
| JMG343 | JMG33/pAM238 | This study | |
| JMG344 | JMG33/pJMG40 | This study | |
| JMG100 | JMG48/pJMG473 | This study | |
| JMG348 | JMG33/pJMG38 | This study | |
| JMG347 | JMG33/pJMG39 | This study | |
| JMG359 | JMG33/pJMG444 | This study | |
| JMG350 | JMG33/pJMG157 | This study | |
| JMG351 | JMG33/pJMG158 | This study | |
| JMG354 | JMG33/pJMG162 | This study | |
| JMG355 | JMG33/pJMG163 | This study | |
| JMG356 | JMG33/pJMG164 | This study | |
| JMG353 | JMG33/pJMG161 | This study | |
| JMG352 | JMG33/pJMG159 | This study | |
| gfp fusion in wild-type background | |||
| EC452 | MC4100 Δ(λattL-lom)::bla lacIqP207-gfp | 30 | |
| EC438 | MC4100 Δ(λattL-lom)::bla lacIqP207-gfp-ftsL | 8 | |
| EC531 | JP313 Δ(λattL-lom)::bla lacIqP207-gfp-ftsL | 8 | |
| JMG287 | MC4100 Δ(λattL-lom)::bla lacIqP207-gfp-LLL | λInCh using pDWS207 derivative pJMG453 | This study |
| JMG441 | JP313 Δ(λattL-lom)::bla lacIqP207-gfp-LLL | λInCh using pDWS207 derivative pJMG453 | This study |
| JMG377 | JP313 Δ(λattL-lom)::bla lacIqP207-gfp-FLL | λInCh using pDWS207 derivative pJMG447 | This study |
| JMG469 | MC4100 Δ(λattL-lom)::bla lacIqP207-gfp-FLL | λInCh using pDWS207 derivative pJMG447 | This study |
| JMG289 | MC4100 Δ(λattL-lom)::bla lacIqP207-gfp-LFL | λInCh using pDWS207 derivative pJMG448 | This study |
| JMG292 | MC4100 Δ(λattL-lom)::bla lacIqP207-gfp-FFL | λInCh using pDWS207 derivative pJMG449 | This study |
| JMG290 | MC4100 Δ(λattL-lom)::bla lacIqP207-gfp-LLF | λInCh using pDWS207 derivative pJMG441 | This study |
| JMG291 | MC4100 Δ(λattL-lom)::bla lacIqP207-gfp-FFF | λInCh using pDWS207 derivative pJMG450 | This study |
| JMG295 | MC4100 Δ(λattL-lom)::bla lacIqP207-gfp-LLI | λInCh using pDWS207 derivative pJMG474 | This study |
| JMG299 | MC4100 Δ(λattL-lom)::bla lacIqP207-gfp-LLΔL | λInCh using pDWS207 derivative pJMG465 | This study |
| JMG300 | MC4100 Δ(λattL-lom)::bla lacIqP207-gfp-LLHinfL | λInCh using pDWS207 derivative pJMG461 | This study |
| JMG371 | JP313 Δ(λattL-lom)::bla lacIqP207-gfp-HLL | λInCh using pDWS207 derivative pJMG455 | This study |
| JMG372 | JP313 Δ(λattL-lom)::bla lacIqP207-gfp-LHL | λInCh using pDWS207 derivative pJMG475 | This study |
| JMG374 | JP313 Δ(λattL-lom)::bla lacIqP207-gfp-HHL | λInCh using pDWS207 derivative pJMG456 | This study |
| JMG373 | JP313 Δ(λattL-lom)::bla lacIqP207-gfp-LHH | λInCh using pDWS207 derivative pJMG458 | This study |
| JMG370 | JP313 Δ(λattL-lom)::bla lacIqP207-gfp-LLH | λInCh using pDWS207 derivative pJMG459 | This study |
| JMG375 | JP313 Δ(λattL-lom)::bla lacIqP207-gfp-HLH | λInCh using pDWS207 derivative pJMG457 | This study |
| JMG376 | JP313 Δ(λattL-lom)::bla lacIqP207-gfp-FtsLHinf | λInCh using pDWS207 derivative pJMG454 | This study |
| JMG303 | MC4100 Δ(λattL-lom)::bla lacIqP207-gfp-LLL(L70H) | λInCh using pDWS207 derivative pJMG466 | This study |
| JMG304 | MC4100 Δ(λattL-lom)::bla lacIqP207-gfp-LLL(L84D) | λInCh using pDWS207 derivative pJMG467 | This study |
| JMG301 | MC4100 Δ(λattL-lom)::bla lacIqP207-gfp-LLGCN4L | λInCh using pDWS207 derivative pJMG462 | This study |
| JMG302 | MC4100 Δ(λattL-lom)::bla lacIqP207-gfp-LLC/EBPL | λInCh using pDWS207 derivative pJMG463 | This study |
| gfp fusions in depletion backgrounds | |||
| JMG366 | JMG531 ftsL::TnphoAL81ΔIS50R(Kanr)/pJMG197 | 8 | |
| JMG367 | JMG441 ftsL::TnphoAL81ΔIS50R(Kanr)/pJMG197 | P1 on LMG145 × JMG441, selecting for Kmr | This study |
| JMG420 | JMG377 ftsL::TnphoAL81ΔIS50R(Kanr)/pJMG197 | P1 on LMG145 × JMG377, selecting for Kmr | This study |
| JMG416 | JMG371 ftsL::TnphoAL81ΔIS50R(Kanr)/pJMG197 | P1 on LMG145 × JMG371, selecting for Kmr | This study |
| JMG418 | JMG373 ftsL::TnphoAL81ΔIS50R(Kanr)/pJMG197 | P1 on LMG145 × JMG373, selecting for Kmr | This study |
| JMG415 | JMG370 ftsL::TnphoAL81ΔIS50R(Kanr)/pJMG197 | P1 on LMG145 × JMG370, selecting for Kmr | This study |
| JMG419 | JMG375 ftsL::TnphoAL81ΔIS50R(Kanr)/pJMG197 | P1 on LMG145 × JMG375, selecting for Kmr | This study |
| JMG422 | JMG376 ftsL::TnphoAL81ΔIS50R(Kanr)/pJMG197 | P1 on LMG145 × JMG376, selecting for Kmr | This study |
| JMG417 | JMG372 ftsL::TnphoAL81ΔIS50R(Kanr)/pJMG197 | P1 on LMG145 × JMG372, selecting for Kmr | This study |
| JMG442 | JMG374 ftsL::TnphoAL81ΔIS50R(Kanr)/pJMG197 | P1 on LMG145 × JMG374, selecting for Kmr | This study |
a
P1 indicates P1 transduction. For example, JMG367 was constructed by infecting JMG441 with P1 lysate made from LMG145.