TABLE 2.
Plasmids used
| Plasmid | Relevant features | Source or reference |
|---|---|---|
| pBAD18 | PBAD arabinose regulation, Ampr | 10 |
| pBAD39 | Arabinose regulation, IPTG depa, lacIq StrepS Ampr | L. M. Guzman, unpublished data |
| pAM238 | Plac regulation, Specr | J. P. Bouché, unpublished data |
| pGHIIA44 | H. influenzae ATCC clone, ftsLHinf Ampr | ATCC |
| pGB017 | C/EBP Ampr | 4 |
| pY88 | GCN4 Ampr | 24 |
| pDSW207 | P207-gfp, Ampr | 30 |
| pDSW236 | pDSW207-ftsL, Ampr | 8 |
| pJMG453 | pDSW207-LLL, Ampr | This study |
| pJMG447 | pDSW207-FLL, Ampr | This study |
| pJMG448 | pDSW207-LFL, Ampr | This study |
| pJMG449 | pDSW207-FFL, Ampr | This study |
| pJMG450 | pDSW207-FFF, Ampr | This study |
| pJMG451 | pDSW207-LLF, Ampr | This study |
| pJMG474 | pDSW207-LLI, Ampr | This study |
| pJMG454 | pDSW207-ftsLHinf, Ampr | This study |
| pJMG455 | pDSW207-HLL, Ampr | This study |
| pJMG456 | pDSW207-HHL, Ampr | This study |
| pJMG475 | pDSW207-LHL, Ampr | This study |
| pJMG457 | pDSW207-HLH, Ampr | This study |
| pJMG458 | pDSW207-LHH, Ampr | This study |
| pJMG459 | pDSW207-LLH, Ampr | This study |
| pJMG461 | pDSW207-LLHinfL, Ampr | This study |
| pJMG462 | pDSW207-LLGCN4, Ampr | This study |
| pJMG463 | pDSW207-LLC/EBPL, Ampr | This study |
| pJMG465 | pDSW207-LLΔL, Ampr | This study |
| pJMG466 | pDSW207-LLL(L70H), Ampr | This study |
| pJMG467 | pDSW207-LLL(L84D), Ampr | This study |
| pJMG197 | ftsL Cmr | 8 |
| pJMG64 | ftsL, IPTG dep, lacIq StrepS Ampr | This study |
| pJMG180 | pBAD18-ftsL, Ampr | 10 |
| pJMG460 | pBAD18-LLxL, Ampr | This study |
| pLD45 | pBAD18-LLL, Ampr | 10 |
| pLD63 | pBAD18-FLL, Ampr | 10 |
| pLD94 | pBAD18-LFL, Ampr | 10 |
| pLD90 | pBAD18-FFL, Ampr | 10 |
| pLD47 | pBAD18-FFF, Ampr | 10 |
| pLD116 | pBAD18-LLI, Ampr | 10 |
| pJMG427 | pBAD18-LLHinfL, Ampr | This study |
| pJMG429 | pBAD18-HLL, Ampr | This study |
| pJMG446 | pBAD18-HLH, Ampr | This study |
| pJMG433 | pBAD18-LHH, Ampr | This study |
| pJMG434 | pBAD18-LLH, Ampr | This study |
| pJMG430 | pBAD18-LHL, Ampr | This study |
| pJMG431 | pBAD18-HHL, Ampr | This study |
| pJMG452 | pBAD18 ftsLHinf, Ampr | This study |
| pJMG464 | pBAD18-LLΔL, Ampr | This study |
| pJMG438 | pBAD18-LLL(L70H), Ampr | This study |
| pJMG439 | pBAD18-LLL(L84D), Ampr | This study |
| pJMG428 | pBAD18-LLGCN4L, Ampr | This study |
| pJMG440 | pBAD18-LLC/EBPL, Ampr | This study |
| pJMG40 | pAM238-LLL, Specr | This study |
| pJMG473 | pAM238-LLxL, Specr | This study |
| pJMG38 | pAM238-FLL, Specr | This study |
| pJMG39 | pAM238-LFL, Specr | This study |
| pJMG444 | pAM238-FFL, Specr | This study |
| pJMG158 | pAM238-LLHinfL, Specr | This study |
| pJMG162 | pAM238-ftsLHinf, Specr | This study |
| pJMG157 | pAM238-LLΔL, Specr | This study |
| pJMG155 | pAM238-LLLΔ, Specr | This study |
| pJMG163 | pAM238-LLL(L70H), Specr | This study |
| pJMG164 | pAM238-LLL(L84D), Specr | This study |
| pJMG161 | pAM238-LLGCN4L, Specr | This study |
| pJMG159 | pAM238-LLC/EBPL, Specr | This study |
a
IPTG dep, IPTG-dependent replication.