TABLE 4.

Localization frequencies of and complementation by GFP-FtsL_Ecol-FtsL_Hinf swap proteins

Construct	Structurea	Localization frequencyb		Complementatione
		No. of cells scored	Band at midcell (%)	Swap not fused to GFPc	Swap fused to GFPd
GFP-LLL		310	75	+++	+++
GFP-LLxL		NDf	ND	+++	ND
GFP-LLHinfL		255	40	+	ND
GFP-HLL		278	82	ND	+++
GFP-HHL		310	76	ND	−
GFP-LHH		268	<1	ND	−
GFP-LLH		319	<1	ND	−
GFP-LHL		308	80	ND	−
GFP-HLH		242	<1	ND	−
GFP-FtsLHinf		273	<1	−	−

^aOpen boxes represent domains of E. coli FtsL (FtsL_Ecol). Shaded boxes denote GFP. Hatched boxes represent domains derived from wild-type H. influenzae FtsL (FtsL_Hinf). E and M indicate borders of the transmembrane domain where restriction sites for EagI and MscI, respectively, have been introduced. X indicates the position of the XhoI site introduced to allow partial swapping inside the region encoding the periplasmic domain of FtsL. In FtsL_Ecol, the former changes a Lys to an Arg and the latter changes an Ala to a Gly. In FtsL_Hinf, the former changes an Asn and a Lys to a Gly and an Arg, respectively, and the latter changes a Thr to a Gly. Diagrams of fusion proteins are not to scale. The cytoplasmic and transmembrane domains of FtsL_Ecol contain 37 and 20 amino acids, respectively, while those from FtsL_Hinf contain 24 and 20 amino acids, respectively. The periplasmic domain of FtsL_Ecol contains 64 amino acids. The periplasmic domain of FtsL_Hinf contains 63 amino acids. 

^bStrains used: GFP-LLL, JMG441; GFP-LL_HinfL, JMG300; GFP-HLL, JMG371; GFP-HHL, JMG374; GFP-LHH, JMG373; GFP-LLH, JMG370; GFP-LHL, JMG372; GFP-HLH, JMG375; GFP-FtsL_Hinf, JMG376. 

^cStrains used: LLL, JMG344; LLxL, JMG100; LL_HinfL, JMG351; FtsL_Hinf, JMG354. 

^dStrains used: GFP-LLL, JMG367; GFP-HLL, JMG416; GFP-HHL, JMG442; GFP-LHH, JMG418; GFP-LLH, JMG415; GFP-LHL, JMG417; GFP-HLH, JMG419; GFP-FtsL_Hinf, JMG422. 

^eFor complementation test, plus signs reflect colony size and minus signs indicate no growth. 

^fND, not determined.