TABLE 5.

Localization frequencies of and complementation by GFP-FtsL mutants

Construct	Structurea	Localization frequencyb		Complementatione
		No. of cells scored	Band at midcell (%)	Swap not fused to GFPc	Swap fused to GFPd
GFP-LLL		342	69	+++	+++
GFP-LLΔL		355		−	ND
GFP-LLL(L70H)		310	17	+	ND
GFP-LLL(L84D)		380	28	+	ND
GFP-LLGCN4L		232	<1	−	ND
GFP-LLC/EBPL		218	<1	−	ND

^aOpen boxes represent domains of E. coli FtsL (FtsL_Ecol). Shaded boxes denote GFP. Hatched boxes represent GCN4 leucine zipper. The checkered box represents C/EBP leucine zipper. E and M indicate borders of the transmembrane domain where restriction sites for EagI and MscI, respectively, have been introduced. X indicates the position of the XhoI site introduced to allow partial swapping inside the region encoding the periplasmic domain of FtsL. In LLL, the former changes a Lys to an Arg, and the latter changes an Ala to a Gly. The cytoplasmic and transmembrane domains of LLL contain 37 and 20 amino acids, respectively. The periplasmic domain of LLL contains 64 amino acids. 

^bStrains used: GFP-LLL, JMG287; GFP-LLΔL, JMG299; GFP-LLL_(L70H), JMG303; GFP-LLL_(L84D), JMG304; GFP-LL_GCN4L, JMG301; GFP-LL_C/EBPL, JMG302. 

^cStrains used: LLL, JMG344; LLΔL, JMG350; LLL_(L70H), JMG355; LLL_(L84D), JMG356; LL_GCN4L, JMG353; LL_C/EBPL, JMG352. 

^dStrain used: GFP-LLL, JMG367. ND, not determined. 

^eFor complementation test, plus signs indicate colony size and minus signs indicate no growth.