TABLE 6.
Coiled-coil-forming potential in FtsL gram-negative and gram-positive homologues
| Organism | Predicted coiled coila | Avg scoreb (%) |
|---|---|---|
| Escherichia coli | 60–90 | 5 |
| Escherichia coli (W81 to Y) | 60–90 | 90c |
| Yersinia pestis | 40–80 | 5 |
| Yersinia pestis (W81 to Y) | 40–80 | 90c |
| Buchnera aphidicola | 35–70 | 80 |
| Haemophilus influenzae | 40–80 | 100 |
| Bacillus subtilis | 60–100 | 100 |
| Streptococcus aureus | 60–120 | 95 |
| Streptococcus pneumoniae | 40–110 | 100 |
| Enterococcus faecalis | 75–115 | 100 |
a
Approximate location of the strongest prediction for coiled coil in FtsL amino acid sequence.
b
Scores were calculated according to the work of Lupas et al. (18) with the COILS program on the ISREC web server (http://www.isrec.isb-sib.ch/software /COILS_form.html). Window length, 21; matrix, MTIDK weighted. For E. coli and Y. pestis FtsL, the value reported is the average obtained with all four matrices used.
c
Coiled-coil propensity prediction: consequence of the replacement of W81 (1) by Y (2) in E. coli FtsL and of W66 (3) by Y (4) in Y. pestis FtsL.