TABLE 7.
Localization frequencies, complementation, and dimerization phenotypes of some of the FtsL swap constructs described in this study
| Construct | Localization frequency (%) | Complementationa | SDS-resistant dimersb |
|---|---|---|---|
| Controls | |||
| FtsL | 72 | +++ | ++++ |
| LLL | 69 | +++ | +++ |
| MalF-E. coli FtsL swaps | |||
| FLL | 70 | − | +++ |
| LFL | <1 | − | − |
| FFL | <1 | − | − |
| H. influenzae-E. coli FtsL swaps | |||
| LLHinfL | 40 | +/− | ++ |
| HLL | 82 | + | + |
| LHL | 80 | − | +/− |
| HHL | 76 | − | +/− |
| HLH | <1 | − | − |
| LHH | <1 | − | − |
| E. coli FtsL mutants | |||
| LLL(L70H) | 17 | +/− | +/− |
| LLL(L84D) | 28 | +/− | +/− |
| E. coli FtsL leucine zipper swap | |||
| LLGCN4L | <1 | − | ++++ |
a
For the complementation test, plus signs reflect colony size, minus signs indicate no growth, and +/− indicates poor growth.
b
The plus signs reflect the propensity to form SDS-resistant dimers. +/−, dimers were not consistently observed in all the experiments.